Secondary metabolites by chemical screening. 21. Clonostachydiol, a novel anthelmintic macrodiolide from the fungus Clonostachys cylindrospora (strain FH-A 6607).

The producing organism, strain FH-A 6607, was isolated from a soil sample collected near Rantepao, Sulawesi Island (Indonesia) and exhibited a characteristic brown to yellow colored mycelium. The typical conidiophores were found to be in close morphological analogy to gliocladium and are arranged in self-contained long rows (Fig. 1). Combined with further taxonomical investigations the strain FH-A 6607 was classified as Clonostachys cylindrospora5). This species belongs to the order of Moniliales, which the colletodiol producer Colletotrichum capsici is to be integrated into the order of Melanconiales, which is more related to the genus Fusarium. The structurally related grahamimycin6) is produced by Cytospora sp. and belongs to a third order of fungi named Sphaeropsidalesn)'. The strain FH-A 6607 was cultivated in a medium containing malt extract 2%, yeast extract 0.2%, glucose 1%, and (NH4)2HPO4 0.05% (pH 6.0 prior to sterilization). Production was